Zhang Xian-Jun

doi:10.7498/aps.73.20241072

Abstract

Efficient photosynthesis reaction is attributed to the flexible energy regulation of two important pigment-protein complexes, i.e. photosystem II (PSII) and photosystem I (PSI). Cryogenic spectral microscopy provides information about the spatial distribution and physiological functional states of photosynthetic components in photosynthetic organisms. Under low temperatures, the uphill energy transfer between pigments is efficiently suppressed so that the temperature-dependent PSI can be well analyzed. Therefore, a cryogenic spectral microscope allows us to discuss the physiological events surrounding PSII and PSI in the independent microscopic zones. This technique can be used to complement the insufficiencies of cryogenic electron microscopy and atomic force microscopy in analyzing the photophysics and photochemistry of photosynthetic species. Historically, cryogenic optical microscopes originated from the desire for single-molecule spectroscopy detection. So far, the combination of optical microscopies and various spectroscopic techniques has expanded the possibility of studying photosynthesis from multiple perspectives. In this paper, the important and recent progress of cryogenic spectral microscopy in the field of natural photosynthesis research is reviewed from two aspects: single-molecule spectroscopy and single-cell spectroscopy, and the advantages of this technique in clarifying the correlation between structure variability and function of pigment-protein complexes, as well as the physiological responses of photosynthetic organisms to variable environments, are also illustrated.

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Zhang Xian-Jun^1,2

Corresponding author: Zhang Xian-Jun, zhang.xianjun.p8@alumni.tohoku.ac.jp

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信号源	主要功能	单细胞检测	单分子探测	低温
吸收光谱	叶绿素分子的电子基态, 绝对分子浓度的评估	◎	×	×
荧光激发光谱	捕光天线蛋白的探测, 能量传递路径的定性分析	◎	◎	◎
荧光发射光谱	两个光系统的表征, 荧光淬灭的表征	◎	◎	◎
荧光寿命	发射分子的激发态动力学表征, 量子收率的测量	◎	◎	◎
自发拉曼光谱	检测弱荧光或非荧光分子, 例如胡萝卜素等	◎	Δ	Δ

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Vol. 73, Issue 22 - 2024-11-20

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