In the field of quantum metrology, an important application is quantum parameter estimation. As the fundamental theory of quantum parameter estimation, quantum Cramér-Rao inequality shows that the variance of parameter estimation is determined by the inverse of quantum Fisher information. Higher quantum Fisher information corresponds to a lower variance, thereby improving the precision of parameter estimation. Quantum Fisher information has been extensively investigated in many aspects of non-relativistic quantum mechanics, including entanglement structure detection, quantum teleportation, quantum phase transition, quantum chaos, and quantum computation. However, there are few researches considering the influence of relativistic effect on quantum Fisher information, and therefore, we attempt to investigate this topic in this work. The relativistic transformation of particle states is employed, and the quantum Fisher information about amplitude parameter $ \theta $ and phase parameter $\varphi $ are investigated in moving reference frame. In this work, the parameters to be estimated are encoded into the spin degree of freedom, and the pure single-qubit state and the pure two-qubit state are both considered. The quantum Fisher information about $ \theta $ and $\varphi $ of single-qubit state and two-qubit state in moving reference frame are numerically calculated, respectively. It can be observed that the quantum Fisher information is associated with rapidity, amplitude parameter, and the ratio of the width to the particle mass ${{{\sigma _r}} \mathord{\left/ {\vphantom {{{\sigma _r}} m}} \right. } m}$ . The quantum Fisher information of the estimated parameters decreases with rapidity increasing for both single-qubit state and two-qubit state. As rapidity approaches infinity, i.e. increases to the speed of light, the quantum Fisher information reaches to a constant which decreases as the ratio ${{{\sigma _r}} \mathord{\left/ {\vphantom {{{\sigma _r}} m}} \right. } m}$ increases. More importantly, for the phase parameter $ \varphi $ , it is observed that the quantum Fisher information of two-qubit state reduces more significantly than that of single-qubit state. While, for the amplitude parameter $\theta $ , the quantum Fisher information of two-qubit state is greater than that of single-qubit state. These results are useful and valuable for improving the precision of parameter estimation under the influence of relativistic effect.