- 必威体育下载

姓名
邮箱
手机号码
标题
留言内容
验证码

Abstract

The biomedical application of high-voltage nanosecond pulsed electric fields (nsPEFs) has become an emerging interdisciplinary research field in recent years. Compared with microsecond and millisecond pulsed electric fields, high-voltage nsPEFs can not only lead the cell membrane structure to polarize and dielectric break down the cell membrane structure, i.e. membrane electroporation, but also penetrate into the cell, triggering off organelle bioelectrical effects such as cytoskeleton depolymerization, intracellular calcium ion release, and mitochondrial membrane potential dissipation. Extensive attention has been attracted from related academic communities. In this article, the following aspects are involved. First, the physical model of high-voltage nsPEFs and its bioelectrical effects on cellular organelles are introduced. Then, the existing researches of the interactions of high-voltage nsPEFs with cytoskeleton, mitochondria, endoplasmic reticulum, cell nucleus and other subcellular structure are reviewed and summarized; the relationship between the influence on cellular organelles by high-voltage nsPEFs and the biological effects such as cell death and intercellular communication is highlighted. Finally, the key technical challenges to high-voltage nsPEFs in biomedical research are condensed, followed by the prospects of future research directions.

Keywords:

Authors and contacts

Corresponding author:Zhuang Jie,jzhuang@sibet.ac.cn

Funds:Project supported by the National Key R&D Program of China (Grant Nos. 2019YFC0119102, 2019YFC0118004, 2020YFC0122301) .

FullText HTML: translate this paragraph

References

[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
[16]
[17]
[18]
[19]
[20]
[21]
[22]
[23]
[24]
[25]
[26]
[27]
[28]
[29]
[30]
[31]
[32]
[33]
[34]
[35]
[36]
[37]
[38]
[39]
[40]
[41]
[42]
[43]
[44]
[45]
[46]
[47]
[48]
[49]
[50]
[51]
[52]
[53]
[54]
[55]
[56]
[57]
[58]
[59]
[60]
[61]
[62]
[63]
[64]
[65]
[66]
[67]
[68]
[69]
[70]
[71]
[72]
[73]
[74]
[75]
[76]
[77]
[78]
[79]
[80]
[81]
[82]
[83]
[84]
[85]
[86]
[87]
[88]
[89]
[90]
[91]
[92]
[93]
[94]
[95]
[96]
[97]
[98]
[99]
[100]
[101]
[102]
[103]
[104]
[105]
[106]
[107]
[108]
[109]
[110]
[111]
[112]
[113]
[114]
[115]
[116]
[117]
[118]
[119]
[120]
[121]
[122]
[123]
[124]
[125]
[126]
[127]
[128]
[129]
[130]
[131]
[132]
[133]
[134]

Cited By

DownLoad: Full-Size Img PowerPoint

细胞器	细胞类型	脉冲宽度/ns	电场强度/(kV·cm^–1)	主要结果概述
细胞骨架	GH3, HeLa^[47]	60	12	肌动蛋白丝变短、变细、碎片化、解聚、收缩、分离, 细胞弹性降低; 微管屈曲、解聚、破碎; 微管聚合速率和聚合数量发生变化; 中间丝破坏; 细胞通透性改变; 细胞肿胀、起泡、细胞质颗粒化; 细胞间通讯受抑制; 细胞骨架的破坏受钙离子调控: 1)高钙离子浓度溶液中, nsPEFs处理会使微管解聚, 破坏肌动蛋白丝; 2) 低钙离子浓度溶液中, nsPEFs处理后微管显示正常结构
	BY-2^[48]	10	33
	Jurkat, HeLa, SV40^[49]	60	15, 60
	CHO-K1^[50]	600	19.2
	Jurkat, U937, CHO-K1^[51]	10	150
	WB-F344^[52]	100	5—35
	U87-MG^[53]	10, 100	44
	CHO^[54]	600	16.2
	GUV^[55]		3—10
	tubulin^[56]	10	20
	U2OS^[57]
	CHO-K1^[58]	10, 60	27.7, 150
	HepG2^[59]	450	8
	B16-F10^[60]	300	12, 18, 26, 40, 60
	WB-F344, WB-Ras^[61]	100	20
	U-937^[62]	60	10
线粒体	Jurkat^[63]	60	0—60	线粒体膜通透性改变, 线粒体的通透性转换孔(MPTP)不可逆过度开放; 线粒体膜电位损失; 线粒体肿胀; 线粒体膜蛋白受影响, 线粒体膜间隙蛋白 Cyt-C, AIF 释放入胞浆; 调控线粒体凋亡途径, Caspase-3表达量增加, Bax 表达量增加; 线粒体释放细胞色素C; 影响线粒体信号传导途径; ATP消耗; 胞内ROS水平升高
	N1-S1^[36]	600	0—80
	Jurkat, U-937^[46]	10	50, 150
	Jurkat^[64]	600	0—60
	HeLa S3^[65]	80	20
	HCT116, NCM460^[66]	10, 600, 800	3, 4, 5
	Jurkat, B10-2^[67]	10—300	≤ 300
	Jurkat, HL-60^[68]	10, 60, 300	150, 60, 25
	Jurkat, HL-60^[34]	10—300	15—60
	Hela^[69]	10, 20, 30, 50	40, 45
	CT-26 tumor cells^[70]	10	22
	MCA205, McA-RH7777, JurkatE6-1^[71]	100	6—25
	4T1^[72]	100	46—54
内质网	Jurkat^[73]	7, 10, 30	25	内质网穿孔、损伤; 钙离子释放, 引发胞内钙离子浓度升高; 内质网应激响应; 免疫原性细胞死亡; 肿瘤细胞内与内质网凋亡相关蛋白Caspase-3的释放量增加; 内质网凋亡信号通路起作用
	Jurkat, HL-60^[74]	60, 300	15—60
	Jurkat, HL-60^[75]	10, 60, 300	26, 40, 60, 150, 300 (10 ns); 16, 26, 40, 60 (60 ns); 40 (300 ns)
	Newly outdated platelet^[76]	300	0—30
	Cardiac cells from rats^[77]	4	10—80
	Jurkat^[15]	60	25, 50, 100
	NG108-15^[78]	4	16.2
	CHO-K1^[79]	60	3.7—30
	U937, CHO-K1, BPAE^[80]	300
	HeLa, HEK293T, C2C12^[40]	7, 10, 20	10—50
	HeLa, HEK293, MEF^[81]	14	10, 20, 25, 30, 40, 50
	MG63^[82]	60	6.7, 13.3, 16.7, 20, 26.7, 33.3
	HeLa, MEF^[83]	14, 70	80, 100 (14 ns), 30, 50, 70, 75 (70 ns)
	Bovine chromaffin cells^[84]	5	170
	B16 F10, EL-4^[39]	200	7
	Hela^[85]	20, 500	100, 20
	Murine secondary oocytes^[86]	10	4—10
	MEF^[87]	60—300	30, 60
	CHO-K1, NG108^[88]	300, 600	3.7, 7.4, 11, 1
细胞核	HL-60^[89]	10, 60	65 (10 ns), 25 (60 ns)	核膜穿孔; DNA双链破坏、DNA片段化; 选择性降低DNA甲基化; 核蛋白复合物改变, 抑制snRNA的生成, 改变亚核结构
	B16 F10^[18,90,91]	300	40
	Jurkat^[92]	10	150
	Jurkat, U-937^[93]	10, 300	2.25, 4.5, 150, 290
	B16F10^[60]	300	0—60
	E4 squamous cell^[94]	300	0—60
	Jurkat^[16]	60	10, 15, 25
	N1-S1^[17]	600	0—80
	N1-S1^[95]	100	50
	CHO^[58]	10, 600	18.2, 27.7, 16.7
	HEK 293^[96]	300	25.5
	HL-60^[36]	80	20
	K562, CT26. WT^[97]	600	50
	HL60, Jurkat, ALL^[98]	10, 60, 300	26, 60, 150, 300
	B10-2, HL-60^[99]	10, 50, 60, 300	26, 60, 75, 150
溶酶体	CHO-K1^[41]	1, 20, 600	16.2	溶酶体去膜化、溶酶体损伤; 溶酶体运动受影响, 高钙离子浓度溶液下, 溶酶体迁移停止;
溶酶体	CHO-K1^[42]	600	16.2	溶酶体去膜化、溶酶体损伤; 溶酶体运动受影响, 高钙离子浓度溶液下, 溶酶体迁移停止;
囊泡	Human eosinophils^[100]	60	36, 53	囊泡穿孔; 诱导细胞外囊泡的释放
囊泡	COS-7^[101]	50	20—300	囊泡穿孔; 诱导细胞外囊泡的释放

DownLoad: CSV

[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
[16]
[17]
[18]
[19]
[20]
[21]
[22]
[23]
[24]
[25]
[26]
[27]
[28]
[29]
[30]
[31]
[32]
[33]
[34]
[35]
[36]
[37]
[38]
[39]
[40]
[41]
[42]
[43]
[44]
[45]
[46]
[47]
[48]
[49]
[50]
[51]
[52]
[53]
[54]
[55]
[56]
[57]
[58]
[59]
[60]
[61]
[62]
[63]
[64]
[65]
[66]
[67]
[68]
[69]
[70]
[71]
[72]
[73]
[74]
[75]
[76]
[77]
[78]
[79]
[80]
[81]
[82]
[83]
[84]
[85]
[86]
[87]
[88]
[89]
[90]
[91]
[92]
[93]
[94]
[95]
[96]
[97]
[98]
[99]
[100]
[101]
[102]
[103]
[104]
[105]
[106]
[107]
[108]
[109]
[110]
[111]
[112]
[113]
[114]
[115]
[116]
[117]
[118]
[119]
[120]
[121]
[122]
[123]
[124]
[125]
[126]
[127]
[128]
[129]
[130]
[131]
[132]
[133]
[134]

[1]	Wang Lei, Ma Xi-Yue, Chen Ke-An, Liu Tao.Low frequency sound absorption performance of large sized active micro-perforated panel absorber in free field. Acta Physica Sinica, 2023, 72(6): 064304.doi:10.7498/aps.72.20222151
[2]	Zhuang Jie, Han Rui, Ji Zhen-Yu, Shi Fu-Kun.Uncertainty in prediction of pulsed field ablation caused by parameter diversity in quantifying conductivity models. Acta Physica Sinica, 2023, 72(14): 147701.doi:10.7498/aps.72.20230203
[3]	.Research progress on biological effects of cell membrane under infrared and terahertz irradiation. Acta Physica Sinica, 2022, (): .doi:10.7498/aps.71.20212030
[4]	Wang Cheng-Yao, Li Xu, Lu Xiao-Yun.Fabrication of cyclic olefin polymer and polydimethylsiloxane co-bonded microfluidic device and its appliactions in terahertz biological effects on intestinal cells. Acta Physica Sinica, 2021, 70(24): 248706.doi:10.7498/aps.70.20211807
[5]	Bo Wen-Fei, Che Rong, Kong Lei, Zhang Ming-Jie, Zhang Xiao-Bo.Research progress of biological effects of cell membrane under infrared and terahertz irradiation. Acta Physica Sinica, 2021, 70(24): 248707.doi:10.7498/aps.70.20212030
[6]	Li Yuan, Li Lin-Bo, Wen Jia-Ye, Ni Zheng-Quan, Zhang Guan-Jun.Initiation of nanosecond-pulsed discharge in water: Electrostriction effect. Acta Physica Sinica, 2021, 70(2): 024701.doi:10.7498/aps.70.20201048
[7]	Yao Jia-Feng, Wan Jian-Fen, Yang Lu, Liu Kai, Chen Bai, Wu Hong-Tao.Electrical characteristics of cells with electrical impedance spectroscopy. Acta Physica Sinica, 2020, 69(16): 163301.doi:10.7498/aps.69.20200601
[8]	Tian Xiao-Fei, Zhang Xin.Biological effects on cells in strong static magnetic field. Acta Physica Sinica, 2018, 67(14): 148701.doi:10.7498/aps.67.20180378
[9]	Wang Xiao-Fa, Zhang Jun-Hong, Gao Zi-Ye, Xia Guang-Qiong, Wu Zheng-Mao.Nanosecond mode-locked Tm-doped fiber laser based on graphene saturable absorber. Acta Physica Sinica, 2017, 66(11): 114209.doi:10.7498/aps.66.114209
[10]	Qiu Ji-Si, Tang Xiong-Xin, Fan Zhong-Wei, Chen Yan-Zhong, Ge Wen-Qi, Wang Hao-Cheng, Liu Hao.High repetition rate and high beam quality joule level Nd: YAG nanosecond laser for Thomson scattering diagnosis. Acta Physica Sinica, 2016, 65(15): 154204.doi:10.7498/aps.65.154204
[11]	Sheng Liang, Li Yang, Wu Jian, Yuan Yuan, Zhao Ji-Zhen, Zhang Mei, Peng Bo-Dong, Hei Dong-Wei.Nanosecond electrical explosion of twisted aluminum wires. Acta Physica Sinica, 2014, 63(20): 205203.doi:10.7498/aps.63.205203
[12]	Li Yuan, Mu Hai-Bao, Deng Jun-Bo, Zhang Guan-Jun, Wang Shu-Hong.Simulational study on streamer discharge in transformer oil under positive nanosecond pulse voltage. Acta Physica Sinica, 2013, 62(12): 124703.doi:10.7498/aps.62.124703
[13]	Yang Hong-Dao, Li Xiao-Hong, Li Guo-Qiang, Yuan Chun-Hua, Tang Duo-Chang, Xu Qin, Qiu Rong, Wang Jun-Bo.Silicon surface microstructures created by 1064 nm Nd∶YAG nanosecond laser. Acta Physica Sinica, 2011, 60(2): 027901.doi:10.7498/aps.60.027901
[14]	Lü Xiao-Gui, Ren Chun-Sheng, Ma Teng-Cai, Zhu Hai-Long, Qian Mu-Yang, Wang De-Zhen.Influence of quartz tube on the nanosecond pulsed discharge in a cone-to-plane air gap. Acta Physica Sinica, 2010, 59(11): 7917-7921.doi:10.7498/aps.59.7917
[15]	Song You-Jian, Hu Ming-Lie, Xie Chen, Chai Lu, Wang Qing-Yue.Approaching 100 nJ pulse energy output from a mode-locked photonic crystal fiber laser. Acta Physica Sinica, 2010, 59(10): 7105-7110.doi:10.7498/aps.59.7105
[16]	Han Jing-Hua, Feng Guo-Ying, Yang Li-Ming, Zhang Qiu-Hui, Jia Jun, Li Gang, Zhu Qi-Hua, Zhou Shou-Huan.Study on the critical free electron density for nanosecond laser pulse focusing in air. Acta Physica Sinica, 2008, 57(10): 6304-6310.doi:10.7498/aps.57.6304
[17]	Hu Jing-Dan, Cai Jing, Chen Jun-Rong, Li Quan, Zhao Ke-Qing.Study on charge transport properties of hexaazatriphenylene and its derivatives. Acta Physica Sinica, 2008, 57(9): 5464-5468.doi:10.7498/aps.57.5464
[18]	Shao Tao, Sun Guang-Sheng, Yan Ping, Gu Chen, Zhang Shi-Chang.Calculation on runaway process of high-energy fast electrons under nanosecond-pulse. Acta Physica Sinica, 2006, 55(11): 5964-5968.doi:10.7498/aps.55.5964
[19]	Tan Xin-Yu, Zhang Duan-Ming, Li Zhi-Hua, Guan Li, Li Li.Target ablation characteristics of thin films during nanosecond pulsed laser deposition in the ablation process. Acta Physica Sinica, 2005, 54(8): 3915-3921.doi:10.7498/aps.54.3915
[20]	Sun Da-Rui, Song Yan-Rong, Zhang Zhi-Gang, Liu Yong-Jun, Chai Lu, Wang Qing-Yue.Compare of characteristics between Martinez and Offner stretchers used in chirped pulse amplifier. Acta Physica Sinica, 2003, 52(4): 870-874.doi:10.7498/aps.52.870

Catalog

Vol. 71, Issue 6 - 2022-03-20

Metrics

Abstract views:9391
PDF Downloads:264
Cited By:0

Search

Article