Measurement-device-independent quantum key distribution (MDI-QKD) is immune to all detection side-channel attacks, thus when combined with the decoy-state method, it can avoid the actual security loophole caused by quasisingle- photon source simultaneously. A practical weak coherent source is used as a quasi-single-photon source in the current MDI-QKD experiments; it may contain percentage of vacuum-and multi-photon pulses. Moreover, in order to study how the performance of the threshold detector affects the quantum bit error rate (QBER), we introduce the quality factor (the ratio of the dark count rate to the detection efficiency) of the threshold detector. Here, through taking into account the weak coherent source, the quality factor of the threshold detector and the reflectivity of beam splitter, we deduce and evaluate the gain, the probability for successful Bell measurement, incorrect Bell measurement when Alice and Bob send pulses with different photon numbers which have a high probability to appear in weak coherent source, and then we obtain QBER in combination with the probabilities of different photon number states, besides, we also do some simulations. The simulations show how QBER varies with the reflectivity of beam splitter and the quality factor of the threshold detector when the average photon numbers per pulse from Alice and Bob are symmetric. Furthermore, the simulations show how QBER varies with the average photon number per pulse from Alice when average photon number per pulse from Bob is 0.1. Result shows that QBER is affected by the reflectivity of beam splitter, but QBER cannot reach the minimum value in Z basis encoding scheme when the average photon numbers per pulse from Alice and Bob are both 0.1 and the reflectivity of beam splitter is 0.5, which is different from X basis encoding and phase encoding. In addition, QBER increases with the increase of the quality factor of the threshold detector, which means that better performance of the threshold detector will reduce QBER. We show that QBER in Z basis encoding reaches the minimum value when reflectivity of beam splitter is 0.5 and there is large difference between in average photon number per pulse between two sides. In conclusion, for QBER, the effect from the reflectivity of beam splitter is equal to average photon numbers from the two arms only in X basis encoding and phase encoding. Our work will provide a reference for setting up a system with better performance.