Polarized Raman spectra of single crystal Bi2ZnOB2O6 have been recorded in the spectral range 101600 cm-1 at room temperature. Factor group analysis was used to obtain the normal modes of vibration of the crystal. The Raman peaks under 300 cm-1 are assigned to external modes, which are related to the rotational and transitional movement of the [BiO6], [ZnO4], [BO4] and [BO3] groups. Compared with the vibrational spectra of the compounds referred, the satisfactory assignment of most of the high-energy modes to vibrations of Bi-O, B-O and Zn-O bonds can be achieved. In particular, the Raman high-frequency peak located at 1407 cm-1 is attributed to the B-O vibration in the [BO3] triangle.