Decoy state has proven to be a very useful method of significantly enhancing the performance of a quantum key distribution (QKD) system with practical light sources. The data-set size in practical QKD protocol is always finite, which will cause statistical fluctuations. The gain and the error rate of the quantum state are analyzed by considering absolutely statistical fluctuation. The relation between key generation rate and the secure communication distance is shown with exchanged quantum signal (N = 106 -1012) by the method of two-decoy-state protocol under the condition that communication wavelength is 1310 nm (or1550 nm). The result indicates that the minimal number of exchanged quantum signals increases obviously with the increase of transmission distance. The secure transmission distance is 135 km under the condition that quantum signal is 1012.