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细胞和组织的力学特性在决定生物功能中起着至关重要的作用, 布里渊光谱技术作为一种黏弹性显微成像方法, 可以无标记、非接触地以高空间分辨率表征样品的力学特征变化. 为了更加灵敏地识别生物系统的微小力学性质差异, 在提高布里渊散射效率的同时, 结合多种黏弹性对比机制进行测量是需要关注的问题. 本文报道了基于脉冲受激布里渊散射的光谱测量方法, 采用脉冲光激发、连续光时域探测的方式, 通过一次时域测量即可得到完整光谱并根据光谱信息反演样品的多种黏弹性参数. 得益于受激散射和时域探测, 实验中以毫秒级的光谱积分时间即可得到信噪比为26 dB的光谱, 弹性纵模的存储模量和损耗模量的平均测量精度分别达0.1%和1%. 基于此方法, 测量并对比了常见液体及聚合物材料的布里渊光谱, 并研究了不同固化阶段的PDMS弹性变化, 与琼脂糖凝胶进行了对比. 最后, 基于多种黏弹性对比机制对6种食用油进行鉴别, 不仅为物质鉴别提供了新的思路, 也拓展了布里渊光谱的测量能力, 提高了黏弹性测量的灵敏性.The mechanical properties of cells and tissues play a crucial role in determining biological functions. As a label-free and non-contact mechanical imaging method, Brillouin spectroscopy can characterize viscoelastic changes in samples with high spatial resolution. To sensitively identify small mechanical differences among biological systems, it is important to improve Brillouin scattering efficiency while combining various viscoelastic contrast mechanisms in measurement. This paper presents a high-speed Brillouin spectroscopy based on impulsive stimulated Brillouin scattering. The acoustic oscillation can be excited in a single shot with a pulsed pump laser and detected by a continuous probe laser in the time domain. This time-domain signal can then be transferred to the frequency-domain Brillouin spectrum with high precision. With this method, various viscoelastic information including sound velocity, sound attenuation coefficient, elastic longitudinal storage modulus, and loss modulus can be obtained simultaneously based on derived spectral information. Owing to stimulated scattering and time-domain detection, spectra with a signal-to-noise ratio of 26 dB can be achieved within a millisecond-level spectral integration time. The average measurement precision for storage modulus and loss modulus of the longitudinal elastic modulus are 0.1% and 1%, respectively. With this method, the Brillouin spectra and viscoelastic parameters of typical liquids and polymer materials are measured and compared, providing a comprehensive reference for viscoelastic parameters. We also study the elastic changes in different curing stages of PDMS and make a comparison of viscoelasticity with agarose gel. Moreover, six edible oils are identified based on various viscoelastic contrast mechanisms, which not only provides a new perspective for material identification but also expands the measurement capabilities of Brillouin spectroscopy and enhances the sensitivity of viscoelasticity measurements.
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Keywords:
- Brillouin scattering/
- viscoelasticity/
- biomechanics
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] -
样品 νB/MHz ΔB/MHz V/(m·s–1) α/(dB·cm–1) M'/MPa M''/MPa 硼酸三甲酯 123.09±0.06 1.54±0.02 1060.0±0.5 26.4±0.3 1028.0±0.9 7.4±0.1 正己烷 127.03±0.03 1.73±0.01 1094.0±0.3 28.7±0.3 790.6±0.4 6.2±0.1 甲醇 130.19±0.02 1.51±0.01 1121.1±0.2 24.4±0.1 994.7±0.3 6.65±0.02 异丙醇 133.87±0.05 2.47±0.04 1152.9±0.4 38.9±0.6 1037.9±0.7 11.1±0.2 乙酸乙酯 134.81±0.04 1.64±0.01 1160.9±0.4 25.7±0.1 1213.4±0.8 8.53±0.04 乙醇 135.34±0.03 1.91±0.02 1165.5±0.3 29.7±0.2 1072.2±0.5 8.7±0.1 丙酮 137.68±0.05 1.58±0.01 1185.7±0.5 24.2±0.1 1102.9±0.8 7.30±0.04 正戊醇 150.17±0.03 2.78±0.05 1293.2±0.3 39.0±0.7 1361.9±0.6 14.6±0.3 油酸 166.85±0.12 7.44±0.06 1436.8±1.0 93.9±0.7 1844.6±2.6 47.5±0.4 角鲨烯 169.87±0.12 4.70±0.07 1462.9±1.0 58.3±0.9 1836.9±2.6 29.4±0.4 二甲基亚砜 173.98±0.08 2.96±0.03 1498.2±0.7 35.9±0.4 2471.4±2.2 24.3±0.2 水 174.69±0.05 2.80±0.05 1504.4±0.4 33.7±0.6 2257.6±1.3 20.9±0.4 
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[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33]
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