In order to explore and develop new crystal materials in the 2.7~3 μm band, Pr,Yb,Ho:GdScO ₃crystal were successfully grown by the Czochralski method for the first time. X-ray diffraction measurement was performed to obtain powder diffraction data. Raman spectra were measured and the vibration peaks were identified. The transmission spectrum, emission spectrum and fluorescence lifetime of Pr,Yb,Ho:GdScO ₃crystal were also characterized. The center of the strongest absorption band is at 966 nm with the half-peak width 90 nm, which comes from the transition of Yb ³⁺: ²F _7/2→ ²F _5/2. The absorption cross section of Yb ³⁺were calculated and the values at 966、973、985 nm are 0.62×10 ^-20, 0.60×10 ^-20and 0.58×10 ^-20cm ²respectively. The maximum emission peak is at 2850 nm and the half-peak width is 70 nm, the lifetimes of Ho ³⁺: ⁵I ₆and ⁵I ₇were measured to be 1094 μs and 56 μs respectively, and the emission cross section at 2850 and 2935 nm were calculated to be 3.6×10 ^-20cm ²and 1.21×10 ^-20cm ². Compared with Yb,Ho:GdScO ₃crystal, the absorption peak of Yb ³⁺and the emission peak were both broadened, which are related to the increase of crystal disorder. The lifetime of the lower energy level decreases significantly. Furthermore, the energy transfer mechanism between Ho ³⁺and Pr ³⁺was analyzed, and the energy transfer efficiency between Ho ³⁺: ⁵I ₇and Pr ³⁺: ³F ₂+ ³H ₆was calculated to be 99%, which is higher than that in other materials. All the results show that Pr,Yb,Ho:GdScO ₃crystal is an excellent 2.7~3 μm laser material, and is easier to achieve laser output than Yb,Ho:GdScO ₃crystal.