In a practical quantum communication system, the security of signal source of continuous-variable quantum key distribution may be jeopardized due to device flaws and hidden attacks. In this paper, an improved scheme for Gaussian-modulated continuous-variable quantum key distribution based on an untrusted entangled source is proposed. In particular, the entanglement source is placed in an untrusted quantum channel to simulate that it is controlled by an eavesdropper, thereby verifying the security of Gaussian-modulated continuous-variable quantum key distribution in a complex environment. This work in detail analyzes the influence of untrusted entanglement source on practical Gaussian-modulated continuous-variable quantum key distribution system, and the numerical simulation shows that the performance of Gaussian-modulated continuous-variable quantum key distribution will dramatically decrease once the entanglement source has moved out of the sender, and it will slightly rise as the untrusted entanglement source slowly moves away from the sender. This paper further introduces two kinds of optical amplifiers, which are phase-sensitive amplifier and phase-insensitive amplifier, to compensate for the imperfection of the coherent detector. These amplifiers are beneficial to enhancing the quantum efficiency of the receiver’s detector. Specifically, the security key rate of Gaussian-modulated continuous-variable quantum key distribution with homodyne detection can be well improved by phase-sensitive amplifier, and the security key rate of Gaussian-modulated continuous-variable quantum key distribution with heterodyne detection can be well improved by phase-insensitive amplifier. To summary, this paper proposes a scheme for Gaussian-modulated continuous-variable quantum key distribution with untrusted entanglement source, experimental results show that the proposed scheme can generate secure quantum keys even if the Gaussian entanglement source is untrusted, and the two optical amplifiers can effectively improve the quantum efficiency of the detector at the receiver. This work aims to promote the practical process of the Gaussian-modulated continuous-variable quantum key distribution system and provide theoretical guidance for the practical implementation and application of the Gaussian-modulated continuous-variable quantum key distribution system.