Asymmetric transmission (AT) metamaterials are extensively studied and applied in the fields of polarization converters and photodiodes. In order to further improve the properties of polarization conversion and unidirectional conduction in the high frequency band and to implement their tunability, the novel chiral electromagnetic metamaterials are studied. By the topology optimization technique, a new type of double-layer L-shaped variant metamaterial structure with excellent asymmetric transmission characteristics is designed. The objective function is to maximize the asymmetric transmission coefficient for the linear polarization wave. The rotationally symmetrical design domain is determined by considering polarization conversion and computation efficiency simultaneously. The design domain of upper layer is divided into two parts which are both the 180° rotationally symmetrical. The design domain of the upper layer and lower layer are the 90° rotationally symmetrical around the xand zaxis respectively. Therefore, the number of design variables is only 18. Asymmetric transmission of linear polarization wave in the K band and Ka band are implemented. Numerical simulation results and experimental results show that the optimized chiral metamaterial has excellent asymmetric transmission characteristics, and its asymmetric transmission coefficient reaches 0.8562 at a frequency of 21.65 GHz and 0.8175 at a frequency of 28.575 GHz. Its asymmetric transmission mechanism is expounded by analyzing the electric field and surface current distribution at the resonance frequency. Based on the optimized chiral metamatertials, the reasonable geometric parameters are selected and the rotation angle of the metal layer is changed in order to further achieve the tunable AT characteristics. First, the influences of the dielectric substrate layer, the thickness of the metal layer and the side length of the grid on resonance frequency and asymmetric transmission coefficient are analyzed respectively, which provides the basis for the reasonable adjustment of the structural parameters to obtain better asymmetric transmission characteristics. After the reasonable geometric parameters are determined, the rotational angle of the upper metal layer and lower metal layer are changed. The linearly and circularly polarized wave are simultaneously achieved in the K band. In this article, the topology optimization technique is used to design the asymmetric transmission chiral metamaterial structure. The design process has a clear direction. The optimized asymmetric transmission chiral metamaterial has the simple structure type and the easy tunability of its asymmetric transmission characteristics. It can be used widely and easily in the fields of polarization converters and photodiodes. This design method has a broad application prospect in the chiral metamaterial field.