The fragmentation experiment of OCS3+ induced by 56 keV/u Ne4+ ions is performed using reaction microscope, and the corresponding dissociation dynamics is investigated. By detecting the three fragment ions in coincidence, the three-dimensional (3D) momenta of all ions and the corresponding kinetic energy release (KER) distributions are reconstructed. It is found that a peak maximum of the KER distribution is locates at about 25 eV, and a shoulder structure appears around 18 eV. This result is consistent with previous heavy ion experimental results with different perturbation strengths. Taking into account that the KER distribution is related to the initial state population of the OCS3+ parent ions, it can be concluded that the perturbation strength is not a decisive parameter leading to the initial state population of OCS3+ ions. We also reconstruct the Newton diagram and Dalitz plot for the three-body fragmentation of OCS3+ ion, from which the sequential dissociation is distinguished from nonsequential dissociation clearly. By analyzing the kinetic energy of ions from each fragmentation process, we find that the KER peak at 25 eV corresponds to nonsequential dissociation process, but the shoulder at 18 eV arises from both sequential and nonsequential dissociation processes. This phenomenon suggests that the parent OCS3+ ions in ground state and low excitation states tend to fragment through sequential dissociation, while those in high excitation states tend to fragment through nosequential dissociation. Furthermore, we reconstruct the KER distributions in the second fragmentation step of sequential dissociation, whose peak maximum is at 6.2 eV, corresponding to X3, 1+ and 1 metastable states of CO2+ ion. A similar KER distribution is obtained for the second fragmentation step of the OCS4+ ion. By comparing our experimental results with previous ones, we conclude that the origin of sequential dissociation process is the existence of metastable state, and the reconstructed KER in the second step reflects the initial state information about the metastable state.