In this paper, we study the influence of the bifocal lens on the focusing properties of Bessel beam. Based on the generalized Huygens-Fresnel diffraction integral, a more general expression of the light intensity distribution of nonsymmetrical optical system which is described by 4×4 matrix is deduced. The optical field distribution of Bessel beam passing through bifocal lens is given and simulated numerically. The influence of the focal length of the bifocal lens on Bessel beam is analyzed in detail. The generalized expression of the light intensity distribution can involve three cases, i.e., ordinary lens, bifocal lens and cylindrical lens. The greater the astigmation, the poorer the bottle beam quality will be. The ability to imprison microscopic particles decreases.